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b. pertussis strains i176, h921  (BEI Resources)

 
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    BEI Resources b. pertussis strains i176, h921
    Antibodies define four competition groups. BLI competitive binding experiments used with antimouse IgG Fc sensors were loaded with a first antibody with mouse constant domains to 0.3 nm, then with Prn1 to reach baseline, then dipped into well with 100 nM of a second antibody with human constant domains. A , representative competition curves with 2B1-mIgG2a immobilized on the sensors and binding to all other antibodies. B , data for competition between all pairwise combinations of antibodies, summarized as a heat map using the signal obtained from the binding of the second antibody. Darker blue depicts lower binding of the second antibody and more competition; lighter blue depicts higher signal from second antibody and less competition. To determine the effective K d for antibody binding to ( C ) Bordetella pertussis <t>TohamaI</t> and ( D ) D420 cells, antibodies (seven 3-fold serial dilutions from 27 to 0.01 μg/ml) were incubated with log-phase bacteria and analyzed by a Fortessa flow cytometer. Mean and standard deviations are shown from two independent experiments. BLI, biolayer interferometry; IgG, immunoglobulin G.
    B. Pertussis Strains I176, H921, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/b%2E+pertussis+i176/b++pertussis+i176/pmc08931430-189-0-9
    Average 90 stars, based on 1 article reviews
    b. pertussis strains i176, h921 - by Bioz Stars, 2026-10
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    1) Product Images from "Antibodies binding diverse pertactin epitopes protect mice from Bordetella pertussis infection"

    Article Title: Antibodies binding diverse pertactin epitopes protect mice from Bordetella pertussis infection

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2022.101715

    Antibodies define four competition groups. BLI competitive binding experiments used with antimouse IgG Fc sensors were loaded with a first antibody with mouse constant domains to 0.3 nm, then with Prn1 to reach baseline, then dipped into well with 100 nM of a second antibody with human constant domains. A , representative competition curves with 2B1-mIgG2a immobilized on the sensors and binding to all other antibodies. B , data for competition between all pairwise combinations of antibodies, summarized as a heat map using the signal obtained from the binding of the second antibody. Darker blue depicts lower binding of the second antibody and more competition; lighter blue depicts higher signal from second antibody and less competition. To determine the effective K d for antibody binding to ( C ) Bordetella pertussis TohamaI and ( D ) D420 cells, antibodies (seven 3-fold serial dilutions from 27 to 0.01 μg/ml) were incubated with log-phase bacteria and analyzed by a Fortessa flow cytometer. Mean and standard deviations are shown from two independent experiments. BLI, biolayer interferometry; IgG, immunoglobulin G.
    Figure Legend Snippet: Antibodies define four competition groups. BLI competitive binding experiments used with antimouse IgG Fc sensors were loaded with a first antibody with mouse constant domains to 0.3 nm, then with Prn1 to reach baseline, then dipped into well with 100 nM of a second antibody with human constant domains. A , representative competition curves with 2B1-mIgG2a immobilized on the sensors and binding to all other antibodies. B , data for competition between all pairwise combinations of antibodies, summarized as a heat map using the signal obtained from the binding of the second antibody. Darker blue depicts lower binding of the second antibody and more competition; lighter blue depicts higher signal from second antibody and less competition. To determine the effective K d for antibody binding to ( C ) Bordetella pertussis TohamaI and ( D ) D420 cells, antibodies (seven 3-fold serial dilutions from 27 to 0.01 μg/ml) were incubated with log-phase bacteria and analyzed by a Fortessa flow cytometer. Mean and standard deviations are shown from two independent experiments. BLI, biolayer interferometry; IgG, immunoglobulin G.

    Techniques Used: Binding Assay, Incubation, Bacteria, Flow Cytometry

    Antipertactin antibodies protect mice from Bordetella pertussis infection. A , Balb/c mice (n = 4) were administered an antibody cocktail (7 μg each of 2E9, 2B1, 1E7, and 1F2) or isotype control (10 μg), all as recombinant antibodies expressed with mouse IgG2a/κ constant domains, intranasally before challenge with a lethal dose (2 × 10 8 cfu) of B. pertussis TohamaI and monitored for 10 days. ∗∗ p < 0.005 determined by Mantel–Cox log-rank test. B , mice (n = 6) were administered an antibody cocktail (7 μg each of 2E9, 2B1, 1E7, and 1F2 mouse IgG2a) or isotype control (10 μg) intranasally before challenge with a sublethal dose (2 × 10 8 cfu) of B. pertussis TohamaI, with lung bacterial colonization levels on day 3 postinfection measured by plating of serially diluted lung homogenate. C , the experiment in B , was repeated with each group receiving the treatment of 1E7, 1F2, 2E9, or isotype (n = 3–5, 10 µg). D , the lung colonization experiment was repeated with mice receiving an isotype control (n = 4; 10 μg) or decreasing concentrations of antibody 2E9 (10, 2, and 0.5 μg). E , the partially protective dosage of 2 μg was used to compare the lung colonization levels of mice receiving treatments of 1E7, 1F2, 2B1, or 2E9 as wildtype Fc mouse IgG2a (n = 5–10) or mouse IgG2a-N297A (n = 4–9) as well as an mIgG2a isotype control (n = 9). Data pooled from two independent experiments. For all panels, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001 determined by one-way ANOVA and Tukey's post hoc test to determine p values between groups using GraphPad software. cfu, colony-forming unit; IgG, immunoglobulin G.
    Figure Legend Snippet: Antipertactin antibodies protect mice from Bordetella pertussis infection. A , Balb/c mice (n = 4) were administered an antibody cocktail (7 μg each of 2E9, 2B1, 1E7, and 1F2) or isotype control (10 μg), all as recombinant antibodies expressed with mouse IgG2a/κ constant domains, intranasally before challenge with a lethal dose (2 × 10 8 cfu) of B. pertussis TohamaI and monitored for 10 days. ∗∗ p < 0.005 determined by Mantel–Cox log-rank test. B , mice (n = 6) were administered an antibody cocktail (7 μg each of 2E9, 2B1, 1E7, and 1F2 mouse IgG2a) or isotype control (10 μg) intranasally before challenge with a sublethal dose (2 × 10 8 cfu) of B. pertussis TohamaI, with lung bacterial colonization levels on day 3 postinfection measured by plating of serially diluted lung homogenate. C , the experiment in B , was repeated with each group receiving the treatment of 1E7, 1F2, 2E9, or isotype (n = 3–5, 10 µg). D , the lung colonization experiment was repeated with mice receiving an isotype control (n = 4; 10 μg) or decreasing concentrations of antibody 2E9 (10, 2, and 0.5 μg). E , the partially protective dosage of 2 μg was used to compare the lung colonization levels of mice receiving treatments of 1E7, 1F2, 2B1, or 2E9 as wildtype Fc mouse IgG2a (n = 5–10) or mouse IgG2a-N297A (n = 4–9) as well as an mIgG2a isotype control (n = 9). Data pooled from two independent experiments. For all panels, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001 determined by one-way ANOVA and Tukey's post hoc test to determine p values between groups using GraphPad software. cfu, colony-forming unit; IgG, immunoglobulin G.

    Techniques Used: Infection, Control, Recombinant, Software

    Antibodies fail to mediate complement lysis in vitro . A , a complement lysis assay was used to assess susceptibility to naive sera by incubating log-phase bacteria in a 15 μl reaction with HBSS and naive baboon sera as a complement source at various concentrations. After a 2 h incubation at 37 °C, surviving bacteria were enumerated by plating serial dilutions on BG plates. Antibody-mediated complement killing of ( B ) TohamaI and ( C ) D420 was assessed as in A , with 10% naive baboon sera as a complement source, and heat-treated convalescent baboon sera or recombinant antibodies were expressed with human IgG1/κ constant domains as an antibody source. The results were normalized with the number of surviving bacteria recovered after incubation with only naive sera set at 100%. Results reported show average of two independent experiments, with two technical replicates each. For each panel, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001 determined by one-way ANOVA and Tukey's post hoc test to determine p values between groups receiving immune sera, isotype, or cocktail to group receiving PBS. BG, background; HBSS, Hank's balanced salt solution; IgG, immunoglobulin G.
    Figure Legend Snippet: Antibodies fail to mediate complement lysis in vitro . A , a complement lysis assay was used to assess susceptibility to naive sera by incubating log-phase bacteria in a 15 μl reaction with HBSS and naive baboon sera as a complement source at various concentrations. After a 2 h incubation at 37 °C, surviving bacteria were enumerated by plating serial dilutions on BG plates. Antibody-mediated complement killing of ( B ) TohamaI and ( C ) D420 was assessed as in A , with 10% naive baboon sera as a complement source, and heat-treated convalescent baboon sera or recombinant antibodies were expressed with human IgG1/κ constant domains as an antibody source. The results were normalized with the number of surviving bacteria recovered after incubation with only naive sera set at 100%. Results reported show average of two independent experiments, with two technical replicates each. For each panel, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001 determined by one-way ANOVA and Tukey's post hoc test to determine p values between groups receiving immune sera, isotype, or cocktail to group receiving PBS. BG, background; HBSS, Hank's balanced salt solution; IgG, immunoglobulin G.

    Techniques Used: Lysis, In Vitro, Bacteria, Incubation, Recombinant

    Related Articles

    other:

    Article Title: Antibodies binding diverse pertactin epitopes protect mice from Bordetella pertussis infection
    Article Snippet: B. pertussis strains TohamaI, D420, H973 (catalog no.: NR-42460; BEI Resources), I176 (catalog no.: NR-42463; BEI Resources), H921 (catalog no.: NR-42457; BEI Resources), B. bronchiseptica RB50, and B. parapertussis 12822 were used in this study.



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    b. pertussis strains i176, h921  (BEI Resources)
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    BEI Resources b. pertussis strains i176, h921
    Antibodies define four competition groups. BLI competitive binding experiments used with antimouse IgG Fc sensors were loaded with a first antibody with mouse constant domains to 0.3 nm, then with Prn1 to reach baseline, then dipped into well with 100 nM of a second antibody with human constant domains. A , representative competition curves with 2B1-mIgG2a immobilized on the sensors and binding to all other antibodies. B , data for competition between all pairwise combinations of antibodies, summarized as a heat map using the signal obtained from the binding of the second antibody. Darker blue depicts lower binding of the second antibody and more competition; lighter blue depicts higher signal from second antibody and less competition. To determine the effective K d for antibody binding to ( C ) Bordetella pertussis <t>TohamaI</t> and ( D ) D420 cells, antibodies (seven 3-fold serial dilutions from 27 to 0.01 μg/ml) were incubated with log-phase bacteria and analyzed by a Fortessa flow cytometer. Mean and standard deviations are shown from two independent experiments. BLI, biolayer interferometry; IgG, immunoglobulin G.
    B. Pertussis Strains I176, H921, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/b%2E+pertussis+i176/b++pertussis+i176/pmc08931430-189-0-9
    Average 90 stars, based on 1 article reviews
    b. pertussis strains i176, h921 - by Bioz Stars, 2026-10
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    b. pertussis i176  (BEI Resources)
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    BEI Resources b. pertussis i176
    Antibodies define four competition groups. BLI competitive binding experiments used with antimouse IgG Fc sensors were loaded with a first antibody with mouse constant domains to 0.3 nm, then with Prn1 to reach baseline, then dipped into well with 100 nM of a second antibody with human constant domains. A , representative competition curves with 2B1-mIgG2a immobilized on the sensors and binding to all other antibodies. B , data for competition between all pairwise combinations of antibodies, summarized as a heat map using the signal obtained from the binding of the second antibody. Darker blue depicts lower binding of the second antibody and more competition; lighter blue depicts higher signal from second antibody and less competition. To determine the effective K d for antibody binding to ( C ) Bordetella pertussis <t>TohamaI</t> and ( D ) D420 cells, antibodies (seven 3-fold serial dilutions from 27 to 0.01 μg/ml) were incubated with log-phase bacteria and analyzed by a Fortessa flow cytometer. Mean and standard deviations are shown from two independent experiments. BLI, biolayer interferometry; IgG, immunoglobulin G.
    B. Pertussis I176, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/b%2E+pertussis+i176/b++pertussis+i176/pmc08931430-189-11-15
    Average 90 stars, based on 1 article reviews
    b. pertussis i176 - by Bioz Stars, 2026-10
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    Antibodies define four competition groups. BLI competitive binding experiments used with antimouse IgG Fc sensors were loaded with a first antibody with mouse constant domains to 0.3 nm, then with Prn1 to reach baseline, then dipped into well with 100 nM of a second antibody with human constant domains. A , representative competition curves with 2B1-mIgG2a immobilized on the sensors and binding to all other antibodies. B , data for competition between all pairwise combinations of antibodies, summarized as a heat map using the signal obtained from the binding of the second antibody. Darker blue depicts lower binding of the second antibody and more competition; lighter blue depicts higher signal from second antibody and less competition. To determine the effective K d for antibody binding to ( C ) Bordetella pertussis TohamaI and ( D ) D420 cells, antibodies (seven 3-fold serial dilutions from 27 to 0.01 μg/ml) were incubated with log-phase bacteria and analyzed by a Fortessa flow cytometer. Mean and standard deviations are shown from two independent experiments. BLI, biolayer interferometry; IgG, immunoglobulin G.

    Journal: The Journal of Biological Chemistry

    Article Title: Antibodies binding diverse pertactin epitopes protect mice from Bordetella pertussis infection

    doi: 10.1016/j.jbc.2022.101715

    Figure Lengend Snippet: Antibodies define four competition groups. BLI competitive binding experiments used with antimouse IgG Fc sensors were loaded with a first antibody with mouse constant domains to 0.3 nm, then with Prn1 to reach baseline, then dipped into well with 100 nM of a second antibody with human constant domains. A , representative competition curves with 2B1-mIgG2a immobilized on the sensors and binding to all other antibodies. B , data for competition between all pairwise combinations of antibodies, summarized as a heat map using the signal obtained from the binding of the second antibody. Darker blue depicts lower binding of the second antibody and more competition; lighter blue depicts higher signal from second antibody and less competition. To determine the effective K d for antibody binding to ( C ) Bordetella pertussis TohamaI and ( D ) D420 cells, antibodies (seven 3-fold serial dilutions from 27 to 0.01 μg/ml) were incubated with log-phase bacteria and analyzed by a Fortessa flow cytometer. Mean and standard deviations are shown from two independent experiments. BLI, biolayer interferometry; IgG, immunoglobulin G.

    Article Snippet: B. pertussis strains TohamaI, D420, H973 (catalog no.: NR-42460; BEI Resources), I176 (catalog no.: NR-42463; BEI Resources), H921 (catalog no.: NR-42457; BEI Resources), B. bronchiseptica RB50, and B. parapertussis 12822 were used in this study.

    Techniques: Binding Assay, Incubation, Bacteria, Flow Cytometry

    Antipertactin antibodies protect mice from Bordetella pertussis infection. A , Balb/c mice (n = 4) were administered an antibody cocktail (7 μg each of 2E9, 2B1, 1E7, and 1F2) or isotype control (10 μg), all as recombinant antibodies expressed with mouse IgG2a/κ constant domains, intranasally before challenge with a lethal dose (2 × 10 8 cfu) of B. pertussis TohamaI and monitored for 10 days. ∗∗ p < 0.005 determined by Mantel–Cox log-rank test. B , mice (n = 6) were administered an antibody cocktail (7 μg each of 2E9, 2B1, 1E7, and 1F2 mouse IgG2a) or isotype control (10 μg) intranasally before challenge with a sublethal dose (2 × 10 8 cfu) of B. pertussis TohamaI, with lung bacterial colonization levels on day 3 postinfection measured by plating of serially diluted lung homogenate. C , the experiment in B , was repeated with each group receiving the treatment of 1E7, 1F2, 2E9, or isotype (n = 3–5, 10 µg). D , the lung colonization experiment was repeated with mice receiving an isotype control (n = 4; 10 μg) or decreasing concentrations of antibody 2E9 (10, 2, and 0.5 μg). E , the partially protective dosage of 2 μg was used to compare the lung colonization levels of mice receiving treatments of 1E7, 1F2, 2B1, or 2E9 as wildtype Fc mouse IgG2a (n = 5–10) or mouse IgG2a-N297A (n = 4–9) as well as an mIgG2a isotype control (n = 9). Data pooled from two independent experiments. For all panels, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001 determined by one-way ANOVA and Tukey's post hoc test to determine p values between groups using GraphPad software. cfu, colony-forming unit; IgG, immunoglobulin G.

    Journal: The Journal of Biological Chemistry

    Article Title: Antibodies binding diverse pertactin epitopes protect mice from Bordetella pertussis infection

    doi: 10.1016/j.jbc.2022.101715

    Figure Lengend Snippet: Antipertactin antibodies protect mice from Bordetella pertussis infection. A , Balb/c mice (n = 4) were administered an antibody cocktail (7 μg each of 2E9, 2B1, 1E7, and 1F2) or isotype control (10 μg), all as recombinant antibodies expressed with mouse IgG2a/κ constant domains, intranasally before challenge with a lethal dose (2 × 10 8 cfu) of B. pertussis TohamaI and monitored for 10 days. ∗∗ p < 0.005 determined by Mantel–Cox log-rank test. B , mice (n = 6) were administered an antibody cocktail (7 μg each of 2E9, 2B1, 1E7, and 1F2 mouse IgG2a) or isotype control (10 μg) intranasally before challenge with a sublethal dose (2 × 10 8 cfu) of B. pertussis TohamaI, with lung bacterial colonization levels on day 3 postinfection measured by plating of serially diluted lung homogenate. C , the experiment in B , was repeated with each group receiving the treatment of 1E7, 1F2, 2E9, or isotype (n = 3–5, 10 µg). D , the lung colonization experiment was repeated with mice receiving an isotype control (n = 4; 10 μg) or decreasing concentrations of antibody 2E9 (10, 2, and 0.5 μg). E , the partially protective dosage of 2 μg was used to compare the lung colonization levels of mice receiving treatments of 1E7, 1F2, 2B1, or 2E9 as wildtype Fc mouse IgG2a (n = 5–10) or mouse IgG2a-N297A (n = 4–9) as well as an mIgG2a isotype control (n = 9). Data pooled from two independent experiments. For all panels, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001 determined by one-way ANOVA and Tukey's post hoc test to determine p values between groups using GraphPad software. cfu, colony-forming unit; IgG, immunoglobulin G.

    Article Snippet: B. pertussis strains TohamaI, D420, H973 (catalog no.: NR-42460; BEI Resources), I176 (catalog no.: NR-42463; BEI Resources), H921 (catalog no.: NR-42457; BEI Resources), B. bronchiseptica RB50, and B. parapertussis 12822 were used in this study.

    Techniques: Infection, Control, Recombinant, Software

    Antibodies fail to mediate complement lysis in vitro . A , a complement lysis assay was used to assess susceptibility to naive sera by incubating log-phase bacteria in a 15 μl reaction with HBSS and naive baboon sera as a complement source at various concentrations. After a 2 h incubation at 37 °C, surviving bacteria were enumerated by plating serial dilutions on BG plates. Antibody-mediated complement killing of ( B ) TohamaI and ( C ) D420 was assessed as in A , with 10% naive baboon sera as a complement source, and heat-treated convalescent baboon sera or recombinant antibodies were expressed with human IgG1/κ constant domains as an antibody source. The results were normalized with the number of surviving bacteria recovered after incubation with only naive sera set at 100%. Results reported show average of two independent experiments, with two technical replicates each. For each panel, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001 determined by one-way ANOVA and Tukey's post hoc test to determine p values between groups receiving immune sera, isotype, or cocktail to group receiving PBS. BG, background; HBSS, Hank's balanced salt solution; IgG, immunoglobulin G.

    Journal: The Journal of Biological Chemistry

    Article Title: Antibodies binding diverse pertactin epitopes protect mice from Bordetella pertussis infection

    doi: 10.1016/j.jbc.2022.101715

    Figure Lengend Snippet: Antibodies fail to mediate complement lysis in vitro . A , a complement lysis assay was used to assess susceptibility to naive sera by incubating log-phase bacteria in a 15 μl reaction with HBSS and naive baboon sera as a complement source at various concentrations. After a 2 h incubation at 37 °C, surviving bacteria were enumerated by plating serial dilutions on BG plates. Antibody-mediated complement killing of ( B ) TohamaI and ( C ) D420 was assessed as in A , with 10% naive baboon sera as a complement source, and heat-treated convalescent baboon sera or recombinant antibodies were expressed with human IgG1/κ constant domains as an antibody source. The results were normalized with the number of surviving bacteria recovered after incubation with only naive sera set at 100%. Results reported show average of two independent experiments, with two technical replicates each. For each panel, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001 determined by one-way ANOVA and Tukey's post hoc test to determine p values between groups receiving immune sera, isotype, or cocktail to group receiving PBS. BG, background; HBSS, Hank's balanced salt solution; IgG, immunoglobulin G.

    Article Snippet: B. pertussis strains TohamaI, D420, H973 (catalog no.: NR-42460; BEI Resources), I176 (catalog no.: NR-42463; BEI Resources), H921 (catalog no.: NR-42457; BEI Resources), B. bronchiseptica RB50, and B. parapertussis 12822 were used in this study.

    Techniques: Lysis, In Vitro, Bacteria, Incubation, Recombinant